Bovine tuberculosis anticorps ELISA kit .

A ɲɛfɔli .

BTB AB ELISA .

Baarakɛcogo laɲininen .: Bovine tuberculose anticorps ELISA detection kit ye ​​bovine tuberculose dantigɛli ye .

Banakisɛfagalan min bɛ bovine ka serum na Mycobacterium bovis in vitro.

Nin sɛgɛsɛgɛli in bɛ kɛ walasa ka bovine tuberculose banakisɛ sɔrɔ. MPB70-MPB83 chimera recombinant kɛra nin sɛgɛsɛgɛli in na.

A ye fɛn minnu kɛ .: Serum .

Fɛn minnu ka kan ka kɛ nka u ma di:

Ji saniyalen .

Piptour .

96-kɔlɔn mikroplate kalanbaga (450nm) .

Timer .

centrifuge (4000rpm) .

Koli masin .

Foro (37 degɛrɛ ) .

Kɔrɔbɔli taabolo & kɔrɔfɔli .

Taabolo 1 .Kɔrɔbɔli kɛcogo .

1.1 Aw bɛ a ko ka ɲɛ .

WOrking Concentration . wAshing jilama:Sani aw ka a kɛ, aw bɛ ko furaji ka kan ka segin so funteni na (20-25 degɛrɛ ) ani ka a lamaga cogo kelen na walasa a ka kɛ kelen ye. O kɔ, aw bɛ ko jilama kɛ siɲɛ 20 ni ji saniyalen ye (misali la, kolijilama 5mL bɛ kɛ ji saniyalen na mililitiri 95), ka a ɲagami kosɛbɛ, ka sɔrɔ ka koliji dilan min bɛ kɛ ka a dilan, o bɛ se ka mara 2-8 la tile 7 kɔnɔ.

1.2 Misali labɛnni .

1 Bagan kuuru joli ta, ka a centrifuged ni 4000rpm ye miniti 10 kɔnɔ, ka ji min bɛ bɔ a la, o lajɛ. Serum fana bɛ se ka wuli ka bɔ a yɛrɛ la ni joli tɛ a la.

2 Aw bɛ serum tacogo 1:100 (add 2ul serum sample fara 198UL misali diluent kan) ka a ɲagami ɲɔgɔn na kosɛbɛ.

KƆLƆSI: Kɔrɔsili juguw ni fura ɲumanw man kan ka dilan.

1.3 Enzyme conjugate labɛnni .

Aw bɛ enzyme conjugate 1:200 (Add 5UL enzyme conjugate(x100) fara 995UL sample diluent kan) ka a ɲagami ɲɔgɔn na kosɛbɛ.

1.4 Kɔrɔbɔli kɛcogo .

1 Aw bɛ microplate label label ta (ka kɛɲɛ ni sɛgɛsɛgɛli hakɛ ye, a bɛ se ka tila ka kɛ siɲɛ caman), ka 100ul kɛ serum diluted la label microplate la, ka kɔlɔn fila kɛ kelen kelen na ka ɲɛsin sɛgɛsɛgɛli juguw ni ɲumanw ma, ani ka 100ul fara a kan walasa ka a kunbɛncogo juguw ni a kɛcogo ɲuman sɔrɔ a kɛcogo ɲuman na. Aw bɛ a sɛgɛsɛgɛli kɛ kɔlɔn kɔnɔ nɔgɔya la, ka a kɛ ni sigilan ye min bɛ kɛ ka a datugu walasa ka a datugu, ka a bila degere 37 la miniti 30 kɔnɔ.

1 Aw bɛ se ka sigilan filimu da wuli, ka furaji kɛ tasa kɔnɔ ka ɲɛ, ka 300μl kɛ baarakɛ hakɛ ko ji la kɔlɔn kelen kelen bɛɛ la, ka sɔrɔ ka ji fili kɔlɔn kɔnɔ. Aw bɛ segin nin wale ninnu kan siɲɛ 5, ka laban ka ja ka a ja papiye saniyalen kan.

3 Aw bɛ 100μl fara kɔlɔn kelen kelen bɛɛ kan, ka tasa datugu, ka a bila degere 37 la miniti 45 kɔnɔ.

1Aw bɛ se ka sigilan filimu da, ka furaji kɛ ka bɔ tasa kɔnɔ ka ɲɛ, ka 300μl kɛ baarakɛ hakɛ ko ji la kɔlɔn kelen kelen bɛɛ la, ka sɔrɔ ka ji fili kɔlɔn kɔnɔ. Aw bɛ segin nin wale ninnu kan siɲɛ 5, ka laban ka ja ka a ja papiye saniyalen kan.

1Add 100μL substrat solution to kelen kelen bɛɛ, ka a fara a kan ka a bila miniti 10 kɔnɔ ni a bɛ se ka kɛ degere 37 ye ka bɔ yeelen na.

1ADD 50μL Stop solution to kelen-kelen bɛɛ la, ka sɔrɔta jateminɛ miniti 5 kɔnɔ.

Taabolo 2nan jaabiw faamuyali .

Kɔlɔn kelen-kelen bɛɛ ka OD450 nafa kalanna kɔlɔn 96 mikroplate kalanbaga kan.

2.1 Tiɲɛni .

OD450NM hakɛ mumɛ hakɛ min bɛ sɔrɔ a la, o tun ye 1000 ye .<0.5 and the average OD450nm value of positive control was ≥1, the experimental results were valid. Otherwise, the test should be repeated.

2.2 Jatebɔcogo .

Sample kelen-kelen bɛɛ la, aw bɛ S/P jatebɔ nin cogo la ni misali latilenni ni kɔrɔsili nafaw ye:

2.3 Jaabiw faamuyali .

Ni S/P ye .<0.25, it is judged as negative.

Ni S/P ka bon ni 0,30 ye walima ni a bɛ bɛn, a bɛ jate koɲuman.

Ni 0,25 ka dɔgɔn walima a bɛ bɛn S/P ma .<0.30, it is judged as suspicious, and suggest to repeat sampling and test one week later.

Tags hot .: Bovine tuberculosis anticorps ELISA Kit, China Bovine tuberculosis anticorps ELISA kit, a dilannikɛlaw, a factory